RNA

Part:BBa_K4811019:Design

Designed by: Kasper Krunderup Jakobsen   Group: iGEM23_DTU-Denmark   (2023-10-03)


PT7-TMS(Kan1)-TT7


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 43
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 43
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 43
    Illegal XhoI site found at 50
    Illegal XhoI site found at 86
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 43
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 43
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

As of 2023, the TMS is a novel BioBrick, and not much is known about the specifics of TMS to RBS binding, as well as how an aptamer insertion into the D-loop will alter this binding, both in presence and absence of the ligand.



Source

The part was ordered as a gBlock from IDT.

The TMS was developed by Avishek Paul during their PhD thesis, see the publication "Modular and Versatile Trans-Encoded Genetic Switches", A. Paul, E. M. Warszawik, M. Loznik, A. J. Boersma, A. Herrmann, Angew. Chem. Int. Ed. 2020, 59, 20328.

References